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GeneticsThis entry was posted on March 16, 2020 by Accu-Scope.
Most compound microscopes come with interchangeable lenses known as objective lenses. Objective lenses come in various magnification powers, with the most common being 4x, 10x, 40x, and 100x, also known as scanning, low power, high power, and (typically) oil immersion objectives, respectively. Let’s take a closer look at each of the different magnifications of objective lenses and when you would use them.
Scanning Objective Lens (4x)
A scanning objective lens provides the lowest magnification power of all objective lenses. 4x is a common magnification for scanning objectives and, when combined with the magnification power of a 10x eyepiece lens, a 4x scanning objective lens gives a total magnification of 40x. The name “scanning” objective lens comes from the fact that they provide observers with about enough magnification for a good overview of the slide, essentially a “scan” of the slide. Some objectives with even lower power are discussed in Specialty Objectives below.
Low Power Objective (10x)
The low power objective lens has more magnification power than the scanning objective lens, and it is one of the most helpful lenses when it comes to observing and analyzing glass slide samples. The total magnification of a low power objective lens combined with a 10x eyepiece lens is 100x magnification, giving you a closer view of the slide than a scanning objective lens without getting too close for general viewing purposes.
High Power Objective Lens (40x)
The high-powered objective lens (also called “high dry” lens) is ideal for observing fine details within a specimen sample. The total magnification of a high-power objective lens combined with a 10x eyepiece is equal to 400x magnification, giving you a very detailed picture of the specimen in your slide.
Oil Immersion Objective Lens (100x)
The oil immersion objective lens provides the most powerful magnification, with a whopping magnification total of 1000x when combined with a 10x eyepiece. But the refractive index of air and your glass slide are slightly different, so a special immersion oil must be used to help bridge the gap. Without adding a drop of immersion oil, the oil immersion objective lens will not function correctly, the specimen will appear blurry, and you will not achieve an ideal magnification or resolution. Oil immersion lenses are also available from some manufacturers in lower magnifications, and provide higher resolution than their "high dry" counterparts.
Specialty Objective Lenses (2x, 50x Oil, 60x and 100x Dry)
There are several other objective lens magnifications available with utility for particular applications. The 2x objective, widely used in pathology, has only ½ the magnification of a 4x scanning lens, thus providing a better overview of the sample on the slide. The 50x oil immersion objective, often used in place of the 40x objective, is used as a gold standard for observing blood smears. The 60x objective, often available in either dry or oil immersion, provides 50% greater magnification than a 40x lens. The 60x dry is sometimes chosen over a 100x oil immersion lens for higher magnification without the need to use immersion oil. Finally the 100x dry objective doesn’t need immersion oil to deliver high magnification (still 1000x when combined with 10x eyepieces). However, the numerical aperture (an indication of resolving power of an objective) of a 100x dry objective is much lower than that of a 100x oil immersion objective and, as a result, the ability of the lens to resolve fine details in the specimen is much lower, too.
It is important to always use the correct immersion media (e.g. air, water, oil, etc.) that is specified by your objective lens.
- The image produced by the wrong immersion media will be blurry. In general, objectives are engineered to "look" through an immersion medium with a particular refractive index (a topic for another article). For example, air has a refractive index of close to 1.0, whereas standard immersion oil has a refractive index of ~1.51.
- You can damage the objective if you use the wrong immersion oil.
If you are interested in buying various types of objective lenses for your microscope in the classroom, laboratory, research facility, or any other purpose, ACCU-SCOPE can provide the products you are looking for. Contact us today to learn more about our objective lenses and other microscope accessories.
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The total magnification of a high-power objective lens combined with a 10x eyepiece is equal to 400x magnification, giving you a very detailed picture of the specimen in your slide.
What will be the total magnification of the ocular lens is 10x and the objective lens is 10x quizlet?
total magnification of both lenses, the objective lens X ocular lens. The ocular eyepiece usually magnifies the image 10X, and the objectives magnify the image 4X, 10X, 40X and 100X. For example, when using the 40X objective and a 10X ocular, the total magnification would be: 4010=400.
What is the total power of a microscope if the ocular lens is 10x and the objective is 40x?
Each objective lens has a different magnification. Multiply the magnification of the eyepiece by the magnification of the objective lens to produce total magnification. For example, a 10X ocular lens and a 40X objective lens will produce a total magnification of 400X (10 x 40 = 400).
What is the total magnification achieved using a 10x objective lens with a 10x eyepiece lens?
The total magnification achieved using a 10× objective lens with a 10× eyepiece lens is 20×. You have successfully focused on an object with the 10x and 40x objective lenses.
What is the total magnification if the ocular lens is 10X and the objective lens is 100X?
Total magnification = 10 X 10 = 100X (this means that the image being viewed will appear to be 100 times its actual size).
What is the total magnification of 10X ocular and 100X oil immersion objective?
1000X
The better the resolving power of the microscope, the closer together two objects can be and still be seen as separate….Terms and Definitions.
| For example: | low power: | (10X)(10X) = 100X |
| high dry: | (40X)(10X) = 400X | |
| oil immersion: | (100X)(10X) = 1000X |
What is the total magnification if the magnification of the eyepiece is 10X and the magnification of the objective lens is 40x?
400x magnification
A microscope’s total magnification is a combination of the eyepieces and the objective lens. For example, a biological microscope with 10x eyepieces and a 40x objective has 400x magnification.
What is a 10x ocular lens?
We all know that 10X means that the objective lens has an effective magnification of ten times life size and when combined in the compound with a 10X ocular lens will give a final magnification of 100X (10 X 10).
How do you calculate total magnification?
To get the total magnification take the power of the objective (4X, 10X, 40x) and multiply by the power of the eyepiece, usually 10X.
What is the total magnification at 10x and 100x?
| Scanning | 4x | 40x |
| Low Power | 10x | 100x |
| High Power | 40x | 400x |
| Oil Immersion | 100x | 1000x |
What is the total magnification when using the 10x ocular lens?
So for 10X objective and 10X ocular, Total magnification = 10 X 10 = 100X (this means that the image being viewed will appear to be 100 times its actual size). Read, more on it here. Also know, what is the total magnification when using a 10x ocular lens and a 40x objective lens?
How to calculate the total magnification of an eyepiece?
To calculate total magnification, find the magnification of both the eyepiece and the objective lenses. The common ocular magnifies ten times, marked as 10x. The standard objective lenses magnify 4x, 10x and 40x.
How many times can you magnify a microscope?
The common ocular magnifies ten times, marked as 10x. The standard objective lenses magnify 4x, 10x and 40x. If the microscope has a fourth objective lens, the magnification will most likely be 100x.
Where are the objective lenses on a microscope?
The ocular or eyepiece is found at the top of the body tube. The objective lens points down toward the object to be magnified. Most microscopes have three or four objective lenses mounted on a rotating nosepiece. Rotating the nosepiece lets the viewer change the magnification.